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Loss of markers of follicular development and maturation after thrombin-induced injury to rat ovary. Thrombin, an enzyme from prothrombin, acts as a proteolytic enzyme and can be locally produced and released into the circulation during clotting events. Although the action of thrombin is well documented, there is little information on the possible effects of this enzyme on ovarian follicle function. In the present study, rats were subjected to a unilateral thrombin injection into the left ovary, and the effects on the contralateral ovary and on the number and morphology of ovarian follicles were examined. The injection of 1 U of thrombin into the left ovary caused a marked reduction in the number of follicles that developed to the antral stage in the contralateral ovary. In addition, the number of primordial follicles present in the left ovary decreased, and although the percentage of growing follicles in the left ovary decreased, most of the remaining growing follicles were atretic. A similar decrease in the number of primordial follicles was observed in the right ovary. In contrast, although injection of 0.1 U of thrombin caused a significant decrease in the number of primordial follicles in the left ovary, the number of developing follicles present in the left ovary increased. The percentages of primordial, growing, atretic, and total follicles present in the right ovary also remained unchanged after thrombin injection. The number of degenerate follicles in the left ovary was increased after thrombin treatment, suggesting that follicles in this ovary were exposed to the enzyme longer than in the untreated ovary. We conclude that thrombin can have a deleterious effect on follicular function in vivo, and that this effect depends on the dose of the enzyme.The present invention relates to a new and distinct cultivar of Bracteantha plant, botanically known as Bracteantha bracteata and hereinafter referred to by the name ‘Bold Lady’. The new Bracteantha is a product of a planned breeding program conducted by the Inventor in Rheinberg, Germany. The objective of the breeding program was to create new compact Bracteantha cultivars with attractive flowers. The new Bracteantha originated from a cross-pollination made by the Inventor in July, 2001 in Rheinberg




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Danea Easyfatt 2009 Ita Crack Full [March-2022]

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